Please note that you can easily find many of these studies yourself, by going to GOOGLE SCHOLAR (if you go to "regular" google you will be BOMBARDED by advertisers on the right column and in the main section). NONE of these advertisers make water that is MEDICALLY CERTIFIED (that's why they need to advertise so much... because these companies have a very difficult time selling them through word of mouth, so they don't produce results good enough to recommend the machine to others).

One clue to tell whether a machine is medically certified (and therefore, is one that makes a difference) is to look at the results people are getting. Tally up the number of actual video testimonials you can find for the different machines (not written testimonials, which are often fake)... find out if the machine is medically certified, find out what the manufacturing cost is, find out how many doctors recommend it. Hopefully this will help you to make an educated decision based on real facts, rather than slick salespeople with unverifiable testimonials. Find out if they have medical certifications, and ask them to see these certifications up close. Ask what their warranty is, and ask if you can see the warranty up close.

Please be aware that the more people click on the ads on the right side of google (not google scholar), the more their webpages will appear in the main section of google (on the first page, and at the top).

Go to GOOGLE SCHOLAR, type in "Electrolyzed Reduced Water" into the search box,

or JUST CLICK HERE.

Here are some of the articles you will find on Electrolyzed Reduced water:

Recent advances in hydrogen research as a therapeutic medical gas

What is Hydroxyl?

Blood Gas Transport (article on making oxygen bio-available - molecular oxygen)

Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-I collagen production and oxidative-stress diminishment in fibroblasts and cell-injury prevention in keratinocytes

Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women: Double-blind, randomized, placebo-controlled, cross-over trial of efficacy and safety

From this page:

Journal of Photochemistry and Photobiology B: Biology

Volume 106, 5 January 2012, Pages 24–33

Shinya Kato ^a^,¹,

Yasukazu Saitoh ^a^,²,

Keizou Iwai ^b,

Nobuhiko Miwa ^a^,

Abstract

Hydrogen-rich electrolyzed warm water (HW) was prepared at 41 °C and exhibited dissolved hydrogen (DH) of 1.13 ppm and an oxidation–reduction potential (ORP) of −741 mV in contrast to below 0.01 ppm and +184 mV for regular warm water (RW). Fibroblasts OUMS-36 and keratinocytes HaCaT were used to examine effects of HW against UVA-ray irradiation. Type-I collagen was synthesized 1.85- to 2.03-fold more abundantly by HW application for 3–5 days than RW in OUMS-36 fibroblasts, and localized preferentially around the nuclei as shown by immunostain. HW application significantly prevented cell death and DNA damages such as nuclear condensation and fragmentation in UVA-irradiated HaCaT keratinocytes as estimated by WST-1 and Hoechst 33342 assays. HW significantly suppressed UVA-induced generation of intracellular superoxide anion radicals in both the cell lines according to NBT assay. Wrinkle repression was clinically assessed using a HW-bathing. Six Japanese subjects were enrolled in a trial of HW-bathing (DH, 0.2–0.4 ppm) every day for 3 months. HW-bathing significantly improved wrinkle in four subjects on the back of neck on 90th day as compared to 0 day. Thus, HW may serve as daily skin care to repress UVA-induced skin damages by ROS-scavenging and promotion of type-I collagen synthesis in dermis.

Highlights

► Hydrogen-rich electrolyzed warm water (HW) has antioxidant activity. ► UVA-derived intracellular reactive oxygen species are scavenged by HW. ► HW promotes the type-I collagen synthesis and suppresses cell death in vitro. ► HW-bathing may serve for daily skin care to repress UVA-induced skin damages.

Keywords

Hydrogen water;

UV-A;

Type-I collagen;

Oxidative stress;

Hydrogen water bathing;

Wrinkle repression

: Corresponding author.

1: Present address: Radioisotope Centre, Osaka City University Graduate School of Medicine, Asahimachi 1-4-3, Abeno-ku, Osaka 545-8585, Japan.

2: Present address: Laboratory of Bioscience & Biotechnology for cell function control, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, Hiroshima 727-0023, Japan.

3: Present address: Department of Radiological Technology, Faculty of Health Sciences, Butsuryo College of Osaka, OtoriKitaMachi 3-33, Nishi-ku, Sakai, Osaka 593-8328, Japan.

PubMed Results

Item 1 of 1

1: Appl Biochem Biotechnol. 2006 Nov;135(2):133-44.Compound (MeSH Keyword), Substance (MeSH Keyword), LinkOut

Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein.

Lee MY, Kim YK, Ryoo KK, Lee YB, Park EJ.

Department of Genetic Engineering, Soonchunhyang University, Asan, Chungnam 336-600, Korea. miyoung@sch.ac.kr

The generation of reactive oxygen species is thought to cause extensive oxidative damage to various biomolecules such as DNA, RNA, and protein. In this study, the preventive, suppressive, and protective effects of in vitro supplementation with electrolyzed-reduced water on H2O2-induced DNA damage in human lymphocytes were examined using a comet assay. Pretreatment, cotreatment, and posttreatment with electrolyzed-reduced water enhanced human lymphocyte resistance to the DNA strand breaks induced by H2O2 in vitro.

Moreover, electrolyzed-reduced water was much more effective than diethylpyrocarbonate-treated water in preventing total RNA degradation at 4 and 25 degrees C. In addition, electrolyzed-reduced water completely prevented the oxidative cleavage of horseradish peroxidase, as determined using sodium dodecyl sulfate-polyacrylamide gels. Enhancement of the antioxidant activity of ascorbic acid dissolved in electrolyzed-reduced water was about threefold that of ascorbic acid dissolved in nonelectrolyzed deionized water, as measured by a xanthine-xanthine oxidase superoxide scavenging assay system, suggesting an inhibitory effect of electrolyzedreduced water on the oxidation of ascorbic acid.

PMID: 17159237 [PubMed - indexed for MEDLINE]

· Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage. [Biochem Biophys Res Commun. 1997]

· The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis. [Biophys Chem. 2004]

Archive > Year 2011, Number 3

Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women: Double-blind, randomized, placebo-controlled, cross-over trial of efficacy and safety

General info

Read full article

INTRODUCTION

The use of performance-enhancing aids has been documented since ancient times and such practices are not reserved for elite or Olympian-level athletes [15]. Since many athletes are looking for ergogenic aids that do not have side effects and cannot be detected during drug testing, nutritional ergogenic aids, including carbohydrates, bicarbonates and dietary antioxidants, are promising alternatives [1]. Athletes who engage in high-intensity exercise such as sprint cycling and swimming along with track events and team sports could be interested in ergogenic aids that buffer against lactic acid [14, 22]. On the other hand, intense physical exercise increases oxidative stress, which leads to enhanced production of free radicals, a factor related to prolonged recovery and exercise-induced fatigue [6] with antioxidant supplementation which can decrease biomarkers of oxidative stress and improve muscular performance in humans [13, 18, 19]. Therefore, a dietary supplement with both buffering and antioxidant effects, could be of particular interest to both recreational and top-level athletes as an effective ergogenic aid. Negative oxidation reduction potential (NORP) alkaline water is often promoted as an antioxidant and anti-aging agent, with clear health benefits yet to be determined. Several recent studies [8, 21] showed anti-microbial activity of electrolyzed oxidizing water against microorganisms relevant in medicine. As an ergogenic aid, NORP alkaline water could be used by athletes in sports such as endurance running to combat the fatiguing effects of lactic acid. Moreover, the strong antioxidant potential of NORP water could be of particular interest for athletes to protect against the damaging effects of free radicals induced by exercise. However, to our best knowledge, no previous cross-sectional or longitudinal study examined the effects of NORP water on human performance. We investigated in a double-blind randomized cross-over trial, firstly, whether intake of the NORP drink improved running performance in young healthy active men and women, secondly, how many participants experienced adverse effects at follow up after this treatment. Therefore, in the current study we tested the hypothesis that an acute (7 days) intake of NORP would reduce the rate of blood lactate accumulation during and after exercise, increase the exhaustion time, increase serum buffering capacity and not increase prevalence of adverse effects.

MATERIALS AND METHODS
SUBJECTS

Both male and female athletes requesting a preparticipation medical examination at the TIMS Exercise Science Centre, Novi Sad, during November 2009, who were experienced in endurance training (> 2 years) and who were between 20 and 30 years of age (24.3 ± 4.1 years), were candidates for inclusion in the study. They were not admitted to the study if any of the following criteria were present: (1) a history of heart disease; (2) musculoskeletal dysfunction; (3) known metabolic disease; (4) smoking; (5) use of any performance-enhancing drugs or dietary supplements within the past 30 days; (6) an impaired response to exercise test; and (7) residence outside the city of Novi Sad, or unwillingness to return for follow-up. All participants were fully informed verbally and in writing about the nature and demands of the study as well as the known health risks. They completed a health history questionnaire, and gave their informed consent regarding their voluntary participation in the study. Upon initial recruitment, eleven (n = 11) participants (9 men and 2 women) met the criteria to take part in the study. All procedures were performed in accordance with the Declaration of Helsinki and the study was approved by the local IRB. The study was carried out at the Exercise Physiology Laboratory, part of Faculty of Sport Sciences and Tourism, Novi Sad, University of Metropolitan, Serbia.

INTERVENTIONS

Participants were randomized in a double-blind, cross-over design to receive the control drink and the NORP drink, with two single-week periods to study the efficacy of the NORP drink (at a dose of 1 L per day by oral administration) according to exercise rehydration guidelines [1]. The NORP drink was supplied in bottles containing water, 2 g NORP, 6 g sucrose, 1-2 mg sodium per dose. The control drink was identically supplied and formulated except that it contained no NORP (Gatorade Sport Drink, San Diego, CA, USA). Subjects self-administered the drink before (30 minutes), during (every 15 min) and after each training session (until 45 min of recovery). The primary endpoint with respect to the efficacy in human performance was the proportion of participants achieving a significant (5%) improvement in running exhaustion time from baseline to 1 week. Additional analyses were done on the blood lactate change during and after exercise and on prevalence of side effects. All testing was conducted at the end of the first and at the end of the second week and the subjects were assessed on the same day with the tests performed in the same order. Participants were instructed to report on adverse effects of supplementation through an open-ended questionnaire at the end of the first and the second weeks of supplementation.

DIETARY CONTROL AND TRAINING

All subjects met a nutritionist who instructed them to maintain their normal dietary pattern throughout the study. During the supplementation regimen all subjects consumed similar standardized diet. Compliance was monitored by analyzing 3-d food records pre- and post-supplementation. Diet records were analyzed for daily caloric intake and composition using food analysis software package. During the trial (7+7 days) all subjects followed a similar training program. Subjects trained for 3 days per week on non-consecutive days. All subjects received a similar personalized training manual with prescribed exercise to be performed. All training sessions were performed at the Faculty’s athletic training facility and monitored by a certified strength and conditioning coach.

EXPERIMENTAL DESIGN

Subjects reported to the laboratory field at 10 a.m. after fast of between 10 and 12 h. Upon entering the laboratory, blood was drawn from the antecubital vein and analyzed for total antioxidant capacity (TAC) by the procedure of chemiluminescence (Boehringer Mannheim GmbH, Germany; cV%=8.2). Fasting blood also was obtained for measurement of glucose and bicarbonates and the sample was sent to the research laboratory, where glucose and bicarbonates were analyzed by standard enzymatic methods and an automated analyzer (Hitachi 704, Tokyo, Japan; cV%=13.0 and cV%=8.5, respectively). For all values, the first reading was discarded and the mean of the next three consecutive readings with a coefficient of variation below 15% was used in the study. A week before the study, the subjects performed a familiarization trial on the treadmill. In the 24 hours before the experiment, the subjects did not participate in any prolonged exercise or drink alcoholic and/or caffeine beverages. Before experimental sessions body mass, height, percentage of body fat, muscle mass and total body hydration from bioimpedance analysis were determined for each subject. Then, the subjects were instrumented for maximal oxygen consumption (VO2MAX) and telemetric heart rate (HR) assessment. Exercise test was performed according to the ramp protocol up to the maximal symptom-tolerated level using a treadmill system (Trackmaster TMX425C, Newton, USA). Gas-exchange data were collected throughout the exercise test using a breath-by-breath metabolic system (Vacu-Med CPX, Ventura, USA) with VO2MAX defined as the highest VO2 achieved during the test with data smoothed before calculating VO2MAX. The heart rate was continuously recorded with a heart rate monitor (Polar S810, Kempele, Finland). The modified rates of perceived exertion (RPE) [17] were monitored during the test (at 3-min intervals), at the end of the test (RPEmax) and after 3 minutes of recovery. During the test (at 8.1 mph running speed) and after the test was completed, the blood was drawn from the fingertip and analyzed immediately for lactate by the procedures of reflectance photometry (Accutrend, USA; cV% = 12.4). The accuracy of the lactate analyzer was checked before each test using standards. The level of blood lactate measured in the third minute after the test was recorded as Lactrrec3, and the level of lactate measured 5 minutes after the test was recorded as Lactrec5.

STATISTICAL ANALYSIS

The data are expressed as Means ± SD. Statistical significance was assessed using Student’s t test for paired samples to evaluate the significance of differences between the values obtained. P values of less than 0.05 were considered to be statistically significant. The data were analyzed using the statistical package SPSS, PC program, version 16.0 (SPSS Inc., USA).

RESULTS

There were no differences in weight, body fat, lean body mass, time to exhaustion, maximal oxygen uptake or ventilatory threshold between the trials (Table 1) (all p > 0.05). Blood glucose and TAC were not affected by supplementation (p > 0.05), while serum bicarbonates were significantly higher after the NORP trial (26.5 ± 2.0 vs. 31.2 ± 1.7 mmol/L; p < 0.05). During the test at 3-min intervals and 3-min post-exercise RPE were similar between both trials (p > 0.05) (Figure 1). Yet, treatment with NORP resulted in significant decrease of RPE at the end of the running test as compared to placebo (p < 0.05). Peak HR, HR at the anaerobic threshold and HRR were not affected by either placebo or NORP (p > 0.05) (Figure 2). Critical HR at the velocity of 8.1 mph during the test was significantly lower in NORP as compared to the placebo trial (p < 0.05). The level of blood lactate sampled at velocity 8.1 mph during the test was significantly lower in the NORP group (p < 0.05) (Figure 3). Post-exercise blood lactates were similar between the trials (p > 0.05). No athletes reported any vexatious side effects of supplementation.

Table 1. Physical and physiological characteristics of the subjects (Mean ± SD)

Note: * indicates significant difference (p < 0.05) between the trials

Figure 1. Rates of perceived exertion (RPE) during the study.

Note: * indicates significant difference (p < 0.05) between the trials

Figure 2. Heart rate (HR) responses during and after the exercise test

Note: * indicates significant difference (p < 0.05) between the trials

Figure 3. Blood lactate (Lac) responses during and after the exercise test

Note: * indicates significant difference (p < 0.05) between the trials

DISCUSSION

This study has provided the first direct analysis of influence of NORP supplementation on human performance indicators and serum antioxidant and buffering capacity in young college athletes. The results of the present study suggest a beneficial effect of NORP supplementation on serum bicarbonates, maximal rate of perceived exertion, heart rate and blood lactate levels at critical running speed (8.1 mph) during the maximal exercise. Treatment with a 2 g oral second-day dose of NORP for 7 days had no significant effect on body mass, body fat, total body hydration and lean body mass nor maximal oxygen uptake in young college athletes, and levels of glucose and total antioxidant capacity were not significantly changed after supplementation of NORP as compared to placebo.

During near-maximal exercise efforts lasting more than 60 s approximately, muscles rely on the anaerobic breakdown of glucose to lactic acid and this metabolic by-product, however, increases muscular [H+] [12). The drop of pH as a result of lactic acid accumulation is thought to inhibit the resynthesis of ATP as well as inhibit muscle contraction [5, 20]. Different buffering agents (i.e. bicarbonates, phosphates, citrates) could increase the body’s capacity to neutralize lactic acid, thereby delaying fatigue during high-intensity exercise [3, 20]. According to the results of the present study, the NORP drink seems to be an effective ergogenic aid since the levels of blood lactates at critical running speed during the maximal running test were lower after NORP intake along with increased bicarbonate levels as compared to the control drink. Similar but non-significant alkalizing effect of NORP has been seen after the exercise test, with blood lactate levels slightly lower at all sampling points for NORP as compared to the control trial. Lower blood lactates at critical speed and improved buffering capacity after NORP intake might indicate better environment for repeated muscle contraction. It could be postulated that the NORP drink buffers [H+] generated by exercise by its alkalinity potential [10]. Yet, since we did not assess exercise performance indicators (e.g. power output) it is not clear if NORP is effective sports ergogenic. However, future studies should further evaluate the pharmacokinetics of NORP along with bioavailability issues, because nowadays there is no scientific evidence about the potential ergogenic effect of NORP. According to the results of the present study, orally administered NORP did increase blood bicarbonates, while other indicators of acid-base balance in arterial blood (e.g. pH, pCO2, hemoglobin) were not assessed for the present study. Due to the fact that NORP exhibits high pH, low dissolved oxygen and extremely high dissolved molecular hydrogen, it seems that increased non-volatile base indicators in the plasma for NORP group arose from ingestion of the alkaline drink. Due to the fact that the intestine is directly involved in acid and/or base generation [21], it appears that NORP has a strong alkalizing effect as a result of absorption of inorganic cations, while protective mechanism of NORP results from active atomic hydrogen with high reductive ability. Although we did not assess pH and pCO2 in the present study, it could be hypothesized that oral intake of alkaline NORP could induce respiratory compensation as the high plasma pH depresses respiration. In consequence, the pCO2 rose and the plasma pH tended to fall towards normal. Yet, since the final correction of alkalosis due to ingestion of base is corrected by renal excretion of the excess base, the analysis of effects of NORP on blood buffering capacity will require assessment of kidney functions (e.g. urine pH, total renal net acid excretion) in prospective studies. Although we examined healthy subjects during the present study, the fact that the appropriate treatment of acute metabolic acidosis (in particular its organic form such as exercise-induced acidosis) has been rather controversial enhances further studies with NORP as a potential anti-acidic treatment strategy and its safe application in clinical patients.

Heart rate (HR) seems to act as a clear indicator of cardiovascular stress induced by exercise. The ease and low-cost of monitoring HR has led to the widespread use of HR as a gauge of relative exercise intensity. Lower HR at similar exercise intensity, induced by training, supplementation or genetic factors, indicates lesser cardiovascular stress and could enhance performance [2]. Due to the fact that HR at the critical speed (8.1 mph) was lower in the NORP trial, it could be postulated that NORP decreases the level of cardiovascular stress response to exercise but the clear mechanism is not known yet. The influence of the NORP drink on blood volume, myocardium contractility and/or stroke volume, and autonomic control of HR should be investigated in the future.

Several research studies suggest that supplemental dietary antioxidants, singularly and in combination, reduce indices of oxidative stress, such as lipid and protein peroxidation [7, 18, 19]. While further studies are needed for specific recommendations for levels of dietary antioxidant intake in athletes, evidence thus far supports that supplemental intake of antioxidants protects against oxidative stress due to exercise and perhaps enhances recovery and minimizes muscle soreness. Although studies in the past showed antioxidant properties of NORP in non-athletic environment [11, 16], during the present study we did not find measurable effects of NORP on the subjects’ antioxidant status assessed by total antioxidant capacity. Measurements of antioxidative status and/or oxidative stress are very complex but generally involve by-products of lipid peroxidation (conjugated dienes, thiobarbituric acid-reactive substances, malondialdehyde, or lipid peroxides). Future studies examining the antioxidant properties of NORP in physically active subjects should be focused on different biomarkers of oxidative stress related to exercise. Furthermore, to connect free radicals with acidosis, some of the biomarkers of oxidative damage need to be measured (LOOH, MDA or F2-isoprostanes).

Perceived exertion is based on the physical sensations a person experiences during physical activity, including increased heart rate, increased respiration or breathing rate, increased sweating, and muscle fatigue [17]. The Borg's perceived exertion scale is a simple method of rating perceived exertion (RPE) and can be used by sport scientists to gauge an athlete's level of intensity in exercise and training [4]. Although RPEs were similar between the trials in the present study during the test and at 3-min post-exercise, the treatment with NORP resulted in significant decrease of RPE at the end of the running test as compared to placebo. The subjects rated the endpoint of exercise as “very hard” (7.8 ± 1.0) after 7-days of NORP administration, while average RPE after administration of the control drink was 8.6 ± 0.9 and the subjects described the test endpoint as “very, very hard”. Due to the fact that RPE illustrates both peripheral and central mechanisms of fatigue [4], it could be hypothesized that the NORP drink decreases the feeling of maximal physical stress affecting both adaptivity of musculoskeletal and reticular activating system of the lower brain to physical exercise [9], which requires further investigation. Assessment of motor cortex excitation, contractile mechanism control and/or sarcolemma excitability after NORP administration is needed to further explore the effects of the NORP drink on fatigue decrement.

Since we had controlled and comparable conditions for all subjects during the study and a double-blind, placebo-controlled design, it is apparent that NORP ingestion had a significant buffering effect for the sample of individuals in the present study. It is noteworthy that in this study we evaluated healthy physically active participants following a regular training program. Yet, it would be premature to conclude that NORP has a performance enhancing effect in all individuals, since no other published studies exist on NORP in the field of biochemistry or nutrition. Dosage and duration of ingestion, purity of the intervention, or the health status of individual may affect the efficacy of NORP administration. A longer supplementation protocol and a higher dosage of the formulation, along with proven bioavailability of the formulation, coupled with monitoring other buffering indicators may be necessary to determine if NORP has a considerable ergogenic effect.

CONCLUSION AND PRACTICAL APPLICATION

This is the first approach to understand the NORP effect in athletes. Intake of a NORP formulation for one week seems to increase serum bicarbonates and reduce the rate of blood lactate accumulation during exercise, maximal rate of perceived exertion and cardiovascular stress at critical running velocity with no significant adverse effects. Future studies should be undertaken in order to fully understand ergogenic potential of negative ionized water.

ACKNOWLEDGEMENTS

The authors declare no conflict of interest.

Study was partially supported by the Serbian Ministry of Science (Grant No. 145082).

Publication of this journal is financially supported by the Ministry of Science, Technology and Development Republic of Serbia.

PubMed Results

Item 1 of 1

1: Appl Biochem Biotechnol. 2006 Nov;135(2):133-44.Compound (MeSH Keyword), Substance (MeSH Keyword), LinkOut

Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein.

Lee MY, Kim YK, Ryoo KK, Lee YB, Park EJ.

Department of Genetic Engineering, Soonchunhyang University, Asan, Chungnam 336-600, Korea. miyoung@sch.ac.kr

PMID: 17159237 [PubMed - indexed for MEDLINE]

· Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage. [Biochem Biophys Res Commun. 1997]

· The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis. [Biophys Chem. 2004]

Cancer research: Alkaline Water (Electrolyzed Reduced Water) 6 recent abstracts EXCERPTS

Anticancer Effect of Alkaline Reduced Water (Full reports available online)

Kyu Jae Lee, Su Ki Kim, Jin Won Kim, Hyun Won Kim, Yonsei University, Wonju, Korea, Sangi University, Korea

Summary:

·Mineral Alkaline Reduced Water (ARW) strengthened immune system.

·Mineral Alkaline Reduced Water suppressed the growth of cancer cells transplanted into mice, demonstrating its anti-cancer effects.

Inhibitory effect of electrolyzed reduced water on tumor angiogenesis Biological & Pharmaceutical Bulletin. 2008 Jan;31(1):19-26 Ye J, Li Y, Hamasaki T, Nakamichi N, Komatsu T, Kashiwagi T, Teruya K, Nishikawa R, Kawahara T, Osada K, Toh K, Abe M, Tian H, Kabayama S, Otsubo K, Morisawa S, Katakura Y, Shirahata S. Graduate School of Systems Life Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan

Suppression of Invasion of Cancer Cells and Angiogenesis by Electrolyzed Reduced Water. From: The Society for In Vitro Biology 2004 World Congress on In Vitro Biology, May 23, 2004 Y. JUN, K. Teruya, Y. Katakura, K. Otsubo*, S. Morisawa*, and S.Shirahata. Dept. of Genetic Resources Technology, Faculty of Agriculture, Kyusyu Univ., 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan *Nihon Trim Co., Ltd. 1-8-34 Oyodonaka, Kita-ku, Osaka 531-0076,
...ERW suppressed the HT1080 cells-induced angiogenesis by human blood endothelial cells, suggesting that ERW may be useful for prevention and treatment of cancer.

Electrolytic reduced water suppresses the invasive metastasis of human fiber sarcoma cells. From US patent #6475371, published Nov. 11, 2002
...Electrolytic reduced water obtained at a cathode electrode side has been found to suppress metastasis of cancer cells. The water had no effects on growth of healthy cells during a one-week test. ... invasive metastasis of HT1080 cells is dramatically reduced in the case of NaOX mix compared to the case of ctrl. This means that the electrolytic reduced water has suppressed the invasive metastasis of the human fiber sarcoma cells.

Suppression of Two-stage Cell Transformation by Electrolyzed Reduced Water/Platinum Nanocolloids.

From: The Society for In Vitro Biology 2004 World Congress on In Vitro Biology, May 22-26, 2004. (presented May 23rd, 2004) R. NISHIKAWA, K. Teruya, Y. Katakura, K. Otsubo, S. Morisawa, and S. Shirahata. Dept. of Genetic Resources Technology, Faculty of Agriculture, Kyusyu Univ., 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan and Nihon Trim Co., Ltd, 1-8-34 Kita-ku, Oyodonaka, Osaka 531-0076, Japan.

Electrolyzed reduced water scavenges active oxygen species and protects DNA from oxidative damage.
Biochem. Biophys. Res. Commun., 234, 269174, 1997Dr. Sanetaka Shirahata, S. et al Graduate school of Genetic Resources Technology , Kyushu University , 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

Anticancer Effect of Alkaline Reduced Water Kyu-Jae LEE1,2, Seung-Kyu PARK1,2, Jae-Won KIM1, Gwang-Young KIM1, Young-Suk RYANG5, Geun-Ha KIM 1, Hyun-Cheol CHO3, Soo-Kie KIM2,3, and Hyun-Won KIM2,41 Dept. of Parasitology, 2 Institute of Basic Medical Sciences, 3 Dept. of Microbiology, 4 Dept. of Biochemistry, Wonju College of Medicine, Yonsei Univ. ( Wonju , Korea) 5Dept. of Biomedical Laboratory Science and Institute of Health Science, College of Health Science, Yonsei Univ. ( Wonju , Korea)...fed with ARW showed tumor growth delay and the survival span was significantly lengthened. ARW also showed the inhibition of metastasis ...Both ROS scavenging effect and immuno-modulation effect might be responsible for anticancer effect of alkaline reduced water.

1: Biochem Biophys Res Commun. 1997 May 8;234(1):269-74. Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOut

Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage.

Shirahata S, Kabayama S, Nakano M, Miura T, Kusumoto K, Gotoh M, Hayashi H, Otsubo K, Morisawa S, Katakura Y.

Institute of Cellular Regulation Technology, Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan. sirahata@grt.kyushu-u.ac.jp

Active oxygen species or free radicals are considered to cause extensive oxidative damage to biological macromolecules, which brings about a variety of diseases as well as aging. The ideal scavenger for active oxygen should be 'active hydrogen'.

'Active hydrogen' can be produced in reduced water near the cathode during electrolysis of water. Reduced water exhibits high pH, low dissolved oxygen (DO), extremely high dissolved molecular hydrogen (DH), and extremely negative redox potential (RP) values. Strongly electrolyzed-reduced water, as well as ascorbic acid, (+)-catechin and tannic acid, completely scavenged O.-2 produced by the hypoxanthine-xanthine oxidase (HX-XOD) system in sodium phosphate buffer (pH 7.0).

The superoxide dismutase (SOD)-like activity of reduced water is stable at 4 degrees C for over a month and was not lost even after neutralization, repeated freezing and melting, deflation with sonication, vigorous mixing, boiling, repeated filtration, or closed autoclaving, but was lost by opened autoclaving or by closed autoclaving in the presence of tungsten trioxide which efficiently adsorbs active atomic hydrogen.

Water bubbled with hydrogen gas exhibited low DO, extremely high DH and extremely low RP values, as does reduced water, but it has no SOD-like activity. These results suggest that the SOD-like activity of reduced water is not due to the dissolved molecular hydrogen but due to the dissolved atomic hydrogen (active hydrogen).

Although SOD accumulated H2O2 when added to the HX-XOD system, reduced water decreased the amount of H2O2 produced by XOD. Reduced water, as well as catalase and ascorbic acid, could directly scavenge H2O2. Reduce water suppresses single-strand breakage of DNA b active oxygen species produced by the Cu(II)-catalyzed oxidation of ascorbic acid in a dose-dependent manner, suggesting that reduced water can scavenge not only O2.- and H2O2, but also 1O2 and .OH.

PMID: 9169001 [PubMed - indexed for MEDLINE]

Anti-diabetic effects of electrolyzed reduced water in streptozotocin-induced and geneticdiabetic mice. Kim MJ, Kim HK.

Department of Obesity management, Graduate School of Obesity Science, Dongduk Women's University, 23-1 Wolkgukdong, Seoul, 136-714, South Korea.

Oxidative stress is produced under diabetic conditions and is likely involved in progression of pancreatic beta-cell dysfunction found in diabetes. Both an increase in reactive oxygen free radical species (ROS) and a decrease in the antioxidant defense mechanism lead to the increase in oxidative stress in diabetes.Electrolyzed reduced water (ERW) with ROS scavenging ability may have a potential effect on diabetic animals, a model for high oxidative stress. Therefore, the present study examined the possible anti-diabetic effect of ERW in two different diabetic animal models.

The genetically diabeticmouse strain C57BL/6J-db/db (db/db) and streptozotocin (STZ)-induced diabetic mouse were used as insulin deficient type 1 and insulin resistant type 2 animal model, respectively. However, ERW fail to affect blood insulin levels in STZ-diabetic mice whereas blood insulin level was markedly increased in genetically diabetic db/db mice. This improved blood glucose control could result from enhanced insulin sensitivity, as well as increased insulin release. The present data suggest that ERW, provided as a drinking water, significantly reduced the blood glucose concentration and improved glucose tolerance in both animal models.ERW may function as an orally effective anti-diabetic agent and merit further studies on its precise mechanism.
PMID: 16945392 [PubMed - indexed for MEDLINE]

CLINICAL STUDY RESULTS SHOWING MICRO-CLUSTERED, HEXAGONAL WATER
HYDRATION IMPROVEMENT IN TYPE 2 DIABETICS

Asia Pac J Clin Nutr. 2004;13(Suppl):S128.
Wang ZY, Zhou ZC, Zhu KN, Wang X, Pan JG, Lorenzen LH, Zhou MC.
Center for Bio-signaling & System Research, New Jersey Institute of Technology, NJ 07102, USA

In 2003 the Chinese Health Care Science and Technology Society organized an international cooperative research project on "Hydration and Health" to compare distilled water (DW) and micro-clustered water (MW). Recent bioelectrical impedance analysis (BIA) studies also showed that diabetics had a lower ratio of intracellular water (ICW) / extracellular water (ECW).

A total 336 type-2 diabetics (plasma glucose level =7.0 mmol/L) from five hospitals were recruited in a randomized, double-blind trial.All the subjects received 250 ml of MW or DW twice daily for 4 weeks. To avoid over-dose absorption, subjects were advised to not take medications within 30 minutes after consumption of the test waters. BIA (RJL, USA) and other clinical markers were performed weekly.

It was observed that MW consumption improved cell water distribution (ICW/ECW), basal metabolism rate (BMR), phase angle (PA) and cell capacitance (CP) during the 4 week testing period. The relative small size of the water cluster may be one of the mechanisms which leads to improved cell structure and function.

In comparison with the rate change from baseline, the P value (MW vs DW) of ICW/ECW, BMR, PA and CP were 0.04, 0.003, 0.005 and 0.003, respectively.

In this study, about 45% of subjects had higher plasma glucose level >8.3 mmol/L).

In comparison with the means of above four BIA measurements at the end of experiment, the P value (MW vs DW) were 0.025, 0.022, 0.007 and 0.009, respectively. Two repeating NMR analysis showed that the half-width of the oxygen (17) NMR spectrum were 64 and 67HZ, respectively, approximating normal saline, plasma and fresh natural spring water, while NMR values of DW and most purified waters exceeded 100 HZ.

Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the l-methyl-4-phenyl-l,2,3,6tetrahydropyridine Mouse Model of Parkinson's Disease

KyotaFujita, Toshihiro Seike, Noriko Yutsudo,Mizuki Ohno,Hidetaka Yamada, Hiroo Yamaguchi, Kunihiko Sakumi, Yukiko Yamakawa, Mizuho A. Kido, Atsushi Takakl, Toshihiko Katafuchl, Yoshinori Tanakas, Yusaku Nakabeppu, Mami Noda

Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan,Division of Neurofunctional Genomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan,Department of Oral Anatomy and Cell Biology, Graduate School of Dental Sciences,Kyushu University, Fukuoka, Japan,Department of Integrative Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan,R&D Center, Home Appliances Manufacturing BusinessUnit, PanasonicElectricWorksCo.,Ltd.,Osaka,Japan

Abstract

It has been shown that molecular hydrogen (H2) acts as a therapeutic antioxidant and suppresses brain injury by buffering the effects of oxidative stress. Chronic oxidative stress causes neurodegenerative diseases such as Parkinson's disease (PD). Here, we show that drinking H2-containing water significantly reduced the loss of dopaminergic neurons in PD model mice using both acute and chronic administration of l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP).

The concentration dependency of H2 showed that H2 as low as 0.08 ppm had almost the same effect as saturated H2 water (1.5 ppm). MPTP induced accumulation of cellular 8-oxoguanine (8-oxoG), a marker of DNA damage, and 4-hydroxynonenal (4-HNE), a marker of lipid peroxidation were significantly decreased in the nigro-striatal dopaminergic pathway in mice drinking Hr containing water, whereas production of superoxide (O2•-) detected by intravascular injection of dihydroethidium (DHE) was not reduced significantly.

Our results indicated that low concentration of H2 in drinking water can reduce oxidative stress in the brain. Thus, drinking H2-containing water may be useful in daily life to prevent or minimize the risk of life stylerelated oxidative stress and neuro-degeneration.

See Hydrogen In Drinking Water.pdf for the paper.

Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice

Ikuroh Ohsawa^a^,^b, Kiyomi Nishimaki^a, Kumi Yamagata^a, Masahiro Ishikawa^a, Shigeo Ohta^a^,^,

^a	Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, NipponMedical School, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211-8533, Japan
^b		The Center of Molecular Hydrogen Medicine, Institute of Development and Aging Sciences, NipponMedical School, Kawasaki 211-8533, Japan

Received 16 October 2008; Available online 6 November 2008.

Abstract

Oxidative stress is implicated in atherogenesis; however most clinical trials with dietary antioxidants failed to show marked success in preventing atherosclerotic diseases. We have found that hydrogen (dihydrogen; H₂) acts as an effective antioxidant to reduce oxidative stress [I. Ohsawa, M. Ishikawa, K. Takahashi, M. Watanabe, K. Nishimaki, K. Yamagata, K. Katsura, Y. Katayama, S, Asoh, S. Ohta, Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals, Nat. Med. 13 (2007) 688–694].

Here, we investigated whether drinking H₂-dissolved water at a saturated level (H₂–water) ad libitum prevents arteriosclerosis using an apolipoprotein E knockout mouse (apoE^−/−), a model of the spontaneous development of atherosclerosis. ApoE^−/− mice drank H₂–water ad libitum from 2 to 6 month old throughout the whole period. Atherosclerotic lesions were significantly reduced by ad libitum drinking of H₂–water (p = 0.0069) as judged by Oil-Red-O staining series of sections of aorta. The oxidative stress level of aorta was decreased. Accumulation of macrophages in atherosclerotic lesions was confirmed. Thus, consumption of H₂-dissolved water has the potential to prevent arteriosclerosis.

Keywords: Antioxidant; ApoE; Arteriosclerosis; Atherogenesis; Dihydrogen; Lifestyle-related disease; Macrophage; Molecular hydrogen; Oxidative stress; Preventive medicine

There are hundreds of medical research studies that have been done to show the effects of Electrolyzed Reduced Water on the human body (as well as in animal studies).

No medical claims are made, and ERW will never replace the guidance of a physician, but feel free to look into these studies and draw your own conclusions.

The medical research studies use either the term Electrolyzed Reduced Water (ERW), Reduced Water, Ionized Water, or Mineral Alkaline Water.

There are many videos you can see, that show the effect of Electrolyzed reduced water on the blood. You can see before and after photos here on the left.

Here are just a few of the medical research studies on this water. Please read all the way down

for cancer research and many others.

Most of the research is from Japan, where this water is used in their hospitals. But, more and more doctors in the US are starting to learn about this technology and get machines for themselves, even though it isn't recognized by the FDA yet. Some US medical schools are now conducting research too.

A Board Member of an American Diabetic Association called Diabetes Support Services briefs Medical and Nursing Students (at a US medical school) about ERW as it is used to drastically improve the circumstances of individuals in clinical settings.

This medical school has a top of the line machine, made with medical parts and a transformer, and is using it to study the water. (Sound quality may not be the best but it is well worth watching!)

This teacher also mentions the "Nordenau Phenomenon."

Japanese hospital footage from a third party using medical grade Electrolyzed Reduced Water machines...these machines are made by an OEM medical manufacturer, servicing Japanese hospitals since the 1970s.

Linked below are specific studies showing the benefits of Electrolyzed ReducedWater from PubMed and other peer-reviewed networks.

Excerpt from science article

http://www.sciencedirect.com/science/article/pii/S0924224411002408

Accumulating evidence has shown that reduced waters are health beneficial and they suppress oxidative stress-related diseases such as diabetes, cancer, arteriosclerosis, neurodegenerative diseases, and the side effects of hemodialysis. The mechanisms of action of reduced water for scavenging ROS are considered to be complicated. ERW contains hydrogen molecules and mineral nanoparticles. Hydrogen molecules and active hydrogen may be new redox regulation factors that can induce the gene expression of antioxidative enzymes. Hydrogen molecules may be converted to active hydrogen by catalyst action of metal nanoparticles to exhibit more potent reducibility. Mineral nanoparticles themselves are new types of multi-functional antioxidants. Mineral hydride nanoparticles, which are H-donors as well as organic antioxidants like ascorbic acid, are also candidates of active agents in reduced waters. NRW may have one or some of the active agents described above. Further investigation on activation methods of water by electricity, magnetic fields or light are likely to contribute to the development of energy-rich waters, which will be beneficial for human health. Reduced water may suppress harmful effects of environmental pollution on the embryo in pregnant women by purifying amniotic fluid and blood.

The next link is a

science text

book containing a summary of the benefits of ERW on major diseases, as well as a bit on the chemistry:

http://books.google.com/books?id=iE-alhelixoC&pg=PA25&lpg=PA25&dq=Suppression+of+Invasion+of+Cancer+Cells+and+Angiogenesis+by+Electrolyzed+Reduced+Water&source=bl&ots=GeDDTrjwxt&sig=8n2O-N_2D466VJ2yf_ML12fCiSY&hl=en&sa=X&ei=IUJ7UMPJKeXm2QWxwICQAg&ved=0CDMQ6AEwAA#v=onepage&q=Suppression%20of%20Invasion%20of%20Cancer%20Cells%20and%20Angiogenesis%20by%20Electrolyzed%20Reduced%20Water&f=false

Anti-cancer effect of ERW:

http://wenku.baidu.com/view/80c7b786b9d528ea81c7798b.html

Studies on ERW reducing oxidative stress:

Electrolyzed Reduced Water Protects Against Oxidative Damage to DNA, RNA and Protein

Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage

The mechanism of the enhanced antioxidant effects against superoxideanion radicals of reduced water produced by electrolysis

Anti-cancer effect of ERW:

http://wenku.baidu.com/view/80c7b786b9d528ea81c7798b.html

Active hydrogen is another property of this water:

http://www.hindawi.com/journals/oximed/2012/353152/

Cancer research: Alkaline Water (Electrolyzed Reduced Water) 6 recent abstracts EXCERPTS

Anticancer Effect of Alkaline Reduced Water

Alkaline vs. tap water	Tap water vs. Alkaline

Kyu Jae Lee, Su Ki Kim, Jin Won Kim, Hyun Won Kim, Yonsei University, Wonju, Korea, Sangi University, Korea Summary:

Mineral Alkaline Reduced Water (ARW) strengthened immune system.
Mineral Alkaline Reduced Water suppressed the growth of cancer cells transplanted into mice, demonstrating its anti-cancer effects.

Suppression of Invasion of Cancer Cells and Angiogenesis by ElectrolyzedReduced Water. From: The Society for In Vitro Biology 2004 World Congress on In Vitro Biology, May 23, 2004 Y. JUN, K. Teruya, Y. Katakura, K. Otsubo*, S. Morisawa*, and S.Shirahata. Dept. of Genetic Resources Technology, Faculty of Agriculture, Kyusyu Univ., 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan *Nihon Trim Co., Ltd. 1-8-34 Oyodonaka, Kita-ku, Osaka 531-0076,
...ERW suppressed the HT1080 cells-induced angiogenesis by human blood endothelial cells, suggesting that ERW may be useful for prevention and treatment of cancer.
Electrolytic reduced water suppresses the invasive metastasis of human fiber sarcoma cells. From US patent #6475371, published Nov. 11, 2002
...Electrolytic reduced water obtained at a cathode electrode side has been found to suppress metastasis of cancer cells. The water had no effects on growth of healthy cells during a one-week test. ... invasive metastasis of HT1080 cells is dramaticallyreduced in the case of NaOX mix compared to the case of ctrl. This means that the electrolytic reduced water has suppressed the invasive metastasis of the human fiber sarcoma cells.

Suppression of Two-stage Cell Transformation by Electrolyzed ReducedWater/Platinum Nanocolloids.
From: The Society for In Vitro Biology 2004 World Congress on In Vitro Biology, May 22-26, 2004. (presented May 23rd, 2004) R. NISHIKAWA, K. Teruya, Y. Katakura, K. Otsubo, S. Morisawa, and S. Shirahata. Dept. of Genetic Resources Technology, Faculty of Agriculture, Kyusyu Univ., 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan and Nihon Trim Co., Ltd, 1-8-34 Kita-ku, Oyodonaka, Osaka 531-0076, Japan.

STOMACH ACID

Prof. Kogure Keizou, Kogure Clinic of Juntendo Hospital

"The stomach is readily upset both by diseases affecting the stomach and by other general illnesses. In addition, any nervous tension or anxiety frequently causes gastric upset, vague symptoms when This information is under some strain.

The important role of antioxidant water in our stomach is to neutralize the secretion and strengthen its functions. Usually, after consuming the antioxidant water for 1 to 3 minutes, the gastric juice increase to 1½ times. For those suffering from hypochlorhydria or achlorhydria ( low in gastric juice ) the presence of antioxidant water will stimulate the stomach cells to secrete more gastric juice. This in turn enhances digestion and absorption of minerals.

However, on the other hand, those with hyperchlorhydria ( high in gastric juice ), the antioxidant water neutralizes the excessive gastric juice.Hence, it does not create any adverse reaction.

According to the medical lecturer from Maeba University, the pH of the gastric secretion will still remain normal when antioxidant water is consumed. This proves that the ability of the antioxidant water is able to neutralize as well as to stimulate the secretion."

----------------------------

Anti-diabetic effects of electrolyzed reduced water in streptozotocin-induced and geneticdiabetic mice.

Kim MJ, Kim HK.

Department of Obesity management, Graduate School of Obesity Science, Dongduk Women's University, 23-1 Wolkgukdong, Seoul, 136-714, South Korea.

Oxidative stress is produced under diabetic conditions and is likely involved in progression of pancreatic beta-cell dysfunction found in diabetes. Both an increase in reactive oxygen free radical species (ROS) and a decrease in the antioxidant defense mechanism lead to the increase in oxidative stress in diabetes.Electrolyzed reduced water (ERW) with ROS scavenging ability may have a potential effect on diabetic animals, a model for high oxidative stress. Therefore, the present study examined the possible anti-diabetic effect of ERW in two different diabetic animal models. The genetically diabeticmouse strain C57BL/6J-db/db (db/db) and streptozotocin (STZ)-induced diabetic mouse were used as insulin deficient type 1 and insulin resistant type 2 animal model, respectively. However, ERW fail to affect blood insulin levels in STZ-diabetic mice whereas blood insulin level was markedly increased in genetically diabetic db/db mice. This improved blood glucose control could result from enhanced insulin sensitivity, as well as increased insulin release. The present data suggest that ERW, provided as a drinking water, significantly reduced the blood glucose concentration and improved glucose tolerance in both animal models.ERW may function as an orally effective anti-diabetic agent and merit further studies on its precise mechanism.
PMID: 16945392 [PubMed - indexed for MEDLINE]

CLINICAL STUDY RESULTS SHOWING MICRO-CLUSTERED, HEXAGONAL WATER
HYDRATION IMPROVEMENT IN TYPE 2 DIABETICS

Asia Pac J Clin Nutr. 2004;13(Suppl):S128.
Wang ZY, Zhou ZC, Zhu KN, Wang X, Pan JG, Lorenzen LH, Zhou MC.Center for Bio-signaling & System Research, New Jersey Institute of Technology, NJ 07102, USA

In 2003 the Chinese Health Care Science and Technology Society organized an international cooperative research project on "Hydration and Health" to compare distilled water (DW) and micro-clustered water (MW). Recent bioelectrical impedance analysis (BIA) studies also showed that diabetics had a lower ratio of intracellular water (ICW) / extracellular water (ECW).

A total 336 type-2 diabetics (plasma glucose level =7.0 mmol/L) from five hospitals were recruited in a randomized, double-blind trial.All the subjects received 250 ml of MW or DW twice daily for 4 weeks. To avoid over-dose absorption, subjects were advised to not take medications within 30 minutes after consumption of the test waters. BIA (RJL, USA) and other clinical markers were performed weekly.

It was observed that MW consumption improved cell water distribution (ICW/ECW), basal metabolism rate (BMR), phase angle (PA) and cell capacitance (CP) during the 4 week testing period. The relative small size of the water cluster may be one of the mechanisms which leads to improved cell structure and function.

In comparison with the rate change from baseline, the P value (MW vs DW) of ICW/ECW, BMR, PA and CP were 0.04, 0.003, 0.005 and 0.003, respectively.

In this study, about 45% of subjects had higher plasma glucose level >8.3 mmol/L).

In comparison with the means of above four BIA measurements at the end of experiment, the P value (MW vs DW) were 0.025, 0.022, 0.007 and 0.009, respectively. Two repeating NMR analysis showed that the half-width of the oxygen (17) NMR spectrum were 64 and 67HZ, respectively, approximating normal saline, plasma and fresh natural spring water, while NMR values of DW and most purified waters exceeded 100 HZ.

Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the l-methyl-4-phenyl-l,2,3,6tetrahydropyridine Mouse Model of Parkinson's Disease

KyotaFujita, Toshihiro Seike, Noriko Yutsudo,Mizuki Ohno,Hidetaka Yamada, Hiroo Yamaguchi, Kunihiko Sakumi, Yukiko Yamakawa, Mizuho A. Kido, Atsushi Takakl, Toshihiko Katafuchl, Yoshinori Tanakas, Yusaku Nakabeppu, Mami Noda

Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan,Division of Neurofunctional Genomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan,Department of Oral Anatomy and Cell Biology, Graduate School of Dental Sciences,Kyushu University, Fukuoka, Japan,Department of Integrative Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan,R&D Center, Home Appliances Manufacturing BusinessUnit, PanasonicElectricWorksCo.,Ltd.,Osaka,Japan

Abstract

It has been shown that molecular hydrogen (H2) acts as a therapeutic antioxidant and suppresses brain injury by buffering the effects of oxidative stress. Chronic oxidative stress causes neurodegenerative diseases such as Parkinson's disease (PD). Here, we show that drinking H2-containing water significantly reduced the loss of dopaminergic neurons in PD model mice using both acute and chronic administration of l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP). The concentration dependency of H2 showed that H2 as low as 0.08 ppm had almost the same effect as saturated H2 water (1.5 ppm). MPTP induced accumulation of cellular 8-oxoguanine (8-oxoG), a marker of DNA damage, and 4-hydroxynonenal (4-HNE), a marker of lipid peroxidation were significantly decreased in the nigro-striatal dopaminergic pathway in mice drinking Hr containing water, whereas production of superoxide (O2•-) detected by intravascular injection of dihydroethidium (DHE) was not reduced significantly.

Our results indicated that low concentration of H2 in drinking water can reduce oxidative stress in the brain. Thus, drinking H2-containing water may be useful in daily life to prevent or minimize the risk of life stylerelated oxidative stress and neuro-degeneration.

Hydrogen In Drinking Water.pdf for the paper.

MORE STUDIES:

Title

Summary

Conference, Collaborators

Year

Inhibiting effect of functional water on formation of fatty acid peroxides

Electrolyzed reduced water was found to inhibit formation of fatty acid peroxides, and antiarteriosclerotic effects are anticipated.

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Sendai)
Yoko Sugihara et al

2002

Protective effect of reduced water against alloxan induced damage to pancreatic ?-cells

Reduced water was found to inhibit damage to pancreatic ?-cells caused by alloxan, which triggers type-1 diabetes, and to promote insulin secretion.

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Sendai)
Makoto Kaminishi et al

2002

Suppressive effect of electrolyzed reduced water on nerve cell death caused by oxidative stress

Electrolyzed reduced water was found to suppress nerve cell death caused by oxidative stress.

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Sendai)
Taichi Kashiwagi et al

2002

Suppressive effect of electrolyzed reduced water on multiplication of human leukemia cells

Electrolyzed reduced water was found to inhibit multiplication of human leukemia cells and to induce differentiation into normal cells.

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Sendai)
Takaaki Komatsu et al

2002

Suppression of lipid peroxidation by electrolyzed reduced water

Electrolyzed reduced water was found to inhibit peroxidation of linoleic acid and cellular membranes.

15th meeting of JAACT, Tokyo
Takeki Hamasaki et al

2002

Electrolyzed reduced water induces differentiation in K562 human leukemia cells.

Electrolyzed reduced water was found to induce megakaryocytic differentiation in human leukemia cells.

15th meeting of JAACT, Tokyo
Takaaki Komatsu et al

2002

Suppression of glutamate-induced neural death of PC12 cells by electrolyzedreduced water

Electrolyzed reduced water was found to inhibit glutamate-induced death of PC12 nerve cells.

15th meeting of JAACT, Tokyo
Taichi Kashiwagi et al

2002

Protective action of reduced water against alloxan-induced cytotoxicity in pancreatic ?-cell

Reduced water was found to provide protection against alloxan-induced cytotoxicity in pancreatic ?-cells.

15th meeting of JAACT, Tokyo
Yuping Li et al

2002

Protective mechanism of reduced water against alloxan-induced pancreatic ?-cell damage: Scavenging effect against reactive oxygen species

By eliminating active oxygen, reduced water was found to provide protection against pancreatic ?-cell damage due to alloxan toxicity.

Cytotechnology, 40, 139–149
Yuping Li et al

2002

Phenotypic changes in tumor cells induced by electrolyzed reduced water

Electrolyzed reduced water was found to inhibit the formation of malignant phenotype colonies by tumor cells

Japan Society for Bioscience, Biotechnology, and Agrochemistry, Joint Conference of Kansai, West Japan, Chugoku, and Shikoku Chapters
Hirofumi Nogami et al

2001

Antidiabetic effectiveness of electrolyzed reduced water and natural reduced water

Both electrolyzed reduced water and natural reduced water were found to promote sugar uptake into muscle tissue and to improve glucose tolerance disorders in model mice with type-2 diabetes.

Japan Society for Bioscience, Biotechnology, and Agrochemistry, Joint Conference of Kansai, West Japan, Chugoku, and Shikoku Chapters
Makoto Kaminishi et al

2001

“Phenotypic changes in tumor cells induced by electrolyzed reduced water”

Electrolyzed reduced water was found to inhibit metastasis and infiltration of cancer cells.

Japanese Association for Animal Cell Technology (Kyoto)
Hirofumi Nogami et al

2001

Suppressive effect of electrolyzed-reduced water on the growth of cancer cells and microorganisms

Electrolyzed reduced water was found to inhibit the proliferation of cancer cells and microorganisms.

17th ESACT Meeting (Sweden)
S. Shirahata et al

2001

Anti-oxidative water improves diabetes

Provides a comprehensive explanation of the effectiveness of anti-oxidative waterin improving diabetes.

17th ESACT Meeting (Sweden)
S. Shirahata et al

2001

Study of cancer-inhibiting effects of electrolyzed reduced water

Using electrolyzed reduced water to suppress the secretion of proteolytic enzymes by cancer cells was found to inhibit metastasis and infiltration of cancer cells.

Japan Society for Bioscience, Biotechnology, and Agrochemistry
Taichi Hara et al

2000

Inhibition of cancerous characteristics by reduced water

Electrolyzed reduced water was found to inhibit characteristics associated with metastasis and infiltration of cancer cells.

Japanese Tissue Culture Association (Okayama)
Kiichiro Teruya et al

2000

Study of cancer-inhibiting effects of electrolyzed reduced water

Electrolyzed reduced water was found to inhibit metastasis and infiltration of cancer cells.

Japan Society for Bioscience, Biotechnology, and Agrochemistry
Taichi Hara et al

1999

Effects of electrolyzed reduced water on sugar uptake signal pathways

Electrolyzed reduced water was found to inhibit the growth of cancer cells and to induce phenotypical changes.

Japan Society for Bioscience, Biotechnology, and Agrochemistry
Masaaki Oda et al

1999

Electrolyzed and natural reduced water exhibit insulin-like activity on glucose uptake into muscle cells and adipocytes

Electrolyzed reduced water was found to promote glucose uptake into muscle cells and adipocytes in a manner similar to insulin.

The 16th meeting of the European Society for Animal Cell Technology
S. Shirahata et al

1999

Effects of electrolyzed reduced water in eliminating active oxygen and protecting against oxidative damage to DNA

Electrolyzed reduced water was found to eliminate superoxide radicals produced in a test tube. It also suppressed DNA damage caused by active oxygen.

Japan Society for Bioscience, Biotechnology, and Agrochemistry
Masaaki Oda et al

1998

Effects of electrolyzed reduced water in inhibiting proliferation of cancer cells

Electrolyzed reduced water was found to inhibit proliferation of cancer cells and to induce phenotypical changes.

Japan Society for Bioscience, Biotechnology, and Agrochemistry
Eri Murakami et al

1998

Telomere shortening in cancer cells by reduced water.

Electrolyzed reduced water was found to induce telomere shortening and deterioration in cancer cells.

The joint international meeting of the Japanese Association for Animal Cell Technology (JAACT) and the European Society for Animal Cell Technology (ESACT)
S. Shirahata et al

1998

Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage.

Electrolyzed reduced water was found to eliminate superoxide radicals produced in a test tube. It also suppressed DNA damage caused by active oxygen.

Biochemical and Biophysical Research Communications, 234, 269-274
S. Shirahata et al

1997

Antioxidative effect of electrolyzed-reduced water supplemented with platinum nanoparticle and effects on parkinson model animal

The 4th meeting of the Japanese Soiety for Functional Water (Hakodate)
Akitami Sato et al

2005

Diabetes prevention through redox regulation by reduced water

The 4th meeting of the Japanese Soiety for Functional Water (Hakodate)
Sanetaka Shirahata et al

2005

Redox regulation and diabetes prevention by reduced water

Japanese Association for Animal Cell Technology (Tokyo) , The joint meeting of west Japan and Hokkaido of Japan Society for Bioscience, Biotechnology, and Agrochemistry (Fukuoka)
Kiichiro Teruya et al

2005

Redox regulation by reduced waters as active hydrogen donors and intracellular ROS scavengers for prevension of diabetes

The 19th European Society for Animal Cell Technology Meeting (Harrogate, UK)
S. Shirahata et al

2005

Induction of caspase-3-dependent apoptosis by electrolyzed reducedwater/platimum nanoparticels in cancer cells

The 19th European Society for Animal Cell Technology Meeting (Harrogate, UK)
T. Hamasaki et al

2005

Analysis of superoxide anion and hydroxyl radical scavenging activities by platinum nanoparticle

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Sapporo)
Takeki Hamasaki et al

2005

Suppressive effect of reduced water with reactive oxygen species scavenging activity on type 1 diabetes mellitus

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Sapporo)
Yuping Li et al

2005

Electrolyzed reduced water supplemented with platinum nanoparticles suppresses promotion of two-stage cell transformation

Cytotechnology
R. Nishikawa et al

2005

Analysis of hydroxyl radical scavenging activity by platinum nanoparticle

The 3rd meeting of the Japanese Soiety for Functional Water (Tokyo)
Takeki Hamasaki et al

2004

Medical application of reduced water as reactive oxygen species scavenger and active hydrogen donor

The 17th Annual Meeting of the Japanese Association for Animal Cell Technology(Nagoya)
S. Shirahata

2004

Platimum nanoparticels in electrolyzed reduced water induces apoptosis in cancer cells

The 17th Annual Meeting of the Japanese Association for Animal Cell Technology(Nagoya)
T. Hamasaki et al

2004

Suppression of two-stage cell transformation by electrolyzed reducedwater/platinum nanocolloids

The 17th Annual Meeting of the Japanese Association for Animal Cell Technology(Nagoya)
R. Nishikawa et al

2004

Suppression of Invasion of Cancer Cells and Angiogenesis by Electrolyzed ReducedWater

2004 World Congress on In Vitro Biology(USA)
Y. Jun et al

2004

Suppression of Two-stage Cell Transformation by Electrolyzed ReducedWater/Platinum Nanocolloids

2004 World Congress on In Vitro Biology(USA)
R. Nishikawa et al

2004

Mechanism for suppressive effect of reduced water with reactive oxygen species scavenging activity on diabetes

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Hiroshima)
Yuping Li et al

2004

Suppression of apoptosis by reduced water in nerve cells

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Hiroshima)
Tenyou Ishikawa et al

2004

Effect of reduction of platinum nanocolloids and electrolyzed-reduced water on nerve cells

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Hiroshima)
Taichi Kashiwagi et al

2004

Induction of cancer cell-specific apoptosis by electrolyzed-reduced water/platinum nanoparticels

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Hiroshima)
Shinsuke Aramaki et al

2004

Reaction kinetics analysis of reactive oxygen species scavenging activity of platinum nanocolloids

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Hiroshima)
Takeki Hamasaki et al

2004

Reduced water functions as active hydrogen donors and ROS scavengers

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Hiroshima)
Sanetaka Shirahata et al

2004

Suppression of carcinogenesis mutation formation at promortion stage byelectrolyzed-reduced water /platinum nanocolloid

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Hiroshima)
Ryuhei Nishikawa et al

2004

Characteristic analysis of reduced waters as active hydrogen donors and reactive oxygen species scavenger

The 2nd meeting of the Japanese Soiety for Functional Water (Gifu)
Sanetaka Shirahata et al

2003

Characteristic analysis of platinum nanocolloids as active hydrogen donors and reactive oxygen species scavenger

The 2nd meeting of the Japanese Soiety for Functional Water (Gifu)
Takeki Hamasaki et al

2003

Suppressive effect of reduced water against alloxan induced type 1 diabetes mellitus

The 2nd meeting of the Japanese Soiety for Functional Water (Gifu)
Yuping Li et al

2003

Effects of Electrolyzed-Reduced Water on the Matrix Metalloproteinase-2 Activities and In Vitro Invasion of Human Fibrosarcoma HT1080 Cells

The 2nd meeting of the Japanese Soiety for Functional Water (Gifu)
J. Ye et al

2003

Induction of apoptosis by platinum nanocolloids/electrolyzed reduced water in cancer cells

The 2nd meeting of the Japanese Soiety for Functional Water (Gifu)
Shinsuke Aramaki et al

2003

Electrolyzed-reduced water protects nerve cells from glutamic acid toxicity

The 2nd meeting of the Japanese Soiety for Functional Water (Gifu)
Taichi Kashiwagi et al

2003

Suppression of apoptosis by reduced water in nerve cells

The 2nd meeting of the Japanese Soiety for Functional Water (Gifu)
Tenyou Ishikawa et al

2003

Suppression of two-stage carcinogenic focus formation by electrolyzed-reducedwater/ platinum nanocolloids

The 2nd meeting of the Japanese Soiety for Functional Water (Gifu)
Ryuhei Nishikawa et al

2003

Function analysis of platinum nanocolloids as active hydrogen donors and reactive oxygen species scavenger

The joint meeting of West Japan and Cyugoku?Sikoku of Japan Society for Bioscience, Biotechnology, and Agrochemistry ,West Japan of Japanese Society of Nutrition and Food Science,West Japan of The Japanese Society for Food Science and Technology (Kagoshima)
Toshifumi Imada et al

2003

Suppressive effect of electrolyzed-reduced water/platinum nanocolloids on proliferation of cancer cells

2003

Suppression of carcinogenic focus formation by electrolyzed-reducedwater/platinum nanocolloids

Japanese Association for Animal Cell Technology (Osaka)
Ryuhei Nishikawa et al

2003

Suppressive effect of electrolyzed-reduced water against nerve cell death induced by oxidative stress

Japanese Association for Animal Cell Technology (Osaka)
Taichi Kashiwagi et al

2003

Scavenging of reactive oxygen species and suppressive effect on cell growth by hydrogen-platinum nano colloids

Japanese Association for Animal Cell Technology (Osaka)
Takeki Hamasaki et al

2003

Suppressive effect of reduced water on pancreatic ?-cells damage caused by oxidative stress

Japanese Society of Nutrition and Food Science (Fukuoka)
Yuping Li et al

2003

Suppressive effect of reduced water on the apoptotic cell death triggered by oxidative stress in pancreatic ? HIT-T15 cells

18th ESACT Meetings(Spain)
S. Shirahata et al

2003

Physiological functions of platinum nanocolloides as scavengers against reactive oxygen species

18th ESACT Meetings(Spain)
T. Hamasaki et al

2003

The inhibition of VEGF expression in human fibrosarcoma HT 1080 cells byelectrolyzed-reduced water

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Kanagawa)
June Ye et al

2003

Effectiveness of platinum nanocolloid in eliminating active oxygen

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Kanagawa)
Takeki Hamasaki et al

2003

Suppressive effect of electrolyzed-reduced water on glutamic acid induced nerve cytotoxicity

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Kanagawa)
Taichi Kashiwagi et al

2003

Suppressive effect of reduced water on the apoptotic cell death triggered by oxidative stress in pancreatic ?-cells

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Kanagawa)
Yuping Li et al

2003

Analysis of mechanisim for differentiation induceded by electrolyzed- reducedwater in K562 human leukemia cells

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Kanagawa)
Takaaki Komatsu et al

2003

The inhibition of VEGF expression in human fibrosarcoma HT1080 cells byelectrolyzed-reduced water

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
June Ye et al

2003

Effectiveness of platinum nanocolloid in eliminating active oxygen

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
Takeki Hamasaki et al

2003

Inhibition of glutamic acid neuron toxicity by electrolyzed reduced water

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
Taichi Kashiwagi et al

2003

Suppressive effect of reduced water on the apoptotic cell death triggered by oxidative stress in pancreatic ?-cells

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
Yuping Li et al

2003

Analysis of electrolyzed reduced water as a differentiation inducing mechanism for K562 human leukemia cells

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
Takaaki Komatsu et al

2003

The inhibition of VEGF expression in human fibrosarcoma HT1080 cells byelectrolyzed-reduced water

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
June Ye et al

2003

Effectiveness of platinum nanocolloid in eliminating active oxygen

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
Takeki Hamasaki et al

2003

Inhibition of glutamic acid neuron toxicity by electrolyzed reduced water

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
Taichi Kashiwagi et al

2003

Suppressive effect of reduced water on the apoptotic cell death triggered by oxidative stress in pancreatic ?-cells

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
Yuping Li et al

2003

Analysis of electrolyzed reduced water as a differentiation inducing mechanism for K562 human leukemia cells

Japan Society for Bioscience, Biotechnology, and Agrochemistry (Tokyo)
Takaaki Komatsu et al

2003

Title

Conference, Collaborators

Year

Suppressive effect of electrolyzed reduced water on angiogenesis and invasion of tumor cells.

The 66th Annual Meeting of the Japanese Cancer Association
S. Shirahata

2007

Research on anti-Parkinson's disease effect by functional food and functional water

The 61st annual meeting of Kyushu?Okinawa of Japanese Society of Nutrition and Food Science
T.Abiru et.al

2007

Research on functional water and functional food with anti-Parkinson's disease

The joint meeting of West Japan and Cyugoku?Sikoku of Japan Society for Bioscience, Biotechnology, and Agrochemistry 2007
T.Abiru et.al

2007

Action mechanism of reduced water for prevention and improvement of oxidative stress-related diseases.

Invited seminar in Karolinska Institute (Stockholm, Sweden)
S. Shirahata

2007

Development of less oxidative hemodialysis solution and it's effect on neutrophil

The 52th Annual Meeting of the Japanese Society for Dialysis Therapy
Masaaki Nakayama et.al.

2007

Effect of Electrolyzed cathodic water (EW) on kidney injury of Dahl salt sensitive rat (DS)

The 50th Annual Meeting of Japanese Society of Nephrology
Wan-Jun Zhu et.al.

2007

Less-oxidative hemodialysis solution rendered by cathode-side application ofelectrolyzed water

Hemodialysis International 2007; 11:322-327
Masaaki Nakayama et al.,

2007

Protective effects of platinum nanoparticles against cellular dysfunction by superoxide anion radical in HeLa cells.

Annual meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
Takeki Hamasaki et al.,

2007

Anti atheloscrelosis effects of Electrolyzed-reduced water.

Annual meeting of Japan Society for Bioscience,
Biotechnology, and Agrochemistry
Masumi Abe et al.,

2007

Evaluation of oxidant and antioxidant activities of various potable waters by ESR.

Annual meeting of Japan Society for Bioscience,
Biotechnology, and Agrochemistry
Kazuko Toh et al.,

2007

Mechanism and function of drinking alkaline electrolyzed water.

The 5th meeting of Japanese society for functional
water (Miyagi Zao)
Sanetaka Shirahata et al.,

2006

Protective effects of platinum nanoparticles against cellular dysfunction by Reactive Oxygen Species.

The 5th meeting of Japanese society for functional
water (Miyagi Zao)
Takeki Hamasaki et al.,

2006

Scavenging activities of superoxide radicals by platinum nanoparticles.

Annual Meeting of the Society of Electron Spin
Science and Technology (Kyoto)
Takeki Hamasaki et al.,

2006

Evaluation of oxidant and antioxidant activities of various potable waters by ESR.

Annual Meeting of the Society of Electron Spin
Science and Technology (Kyoto)
Kazuko Toh et al.,

2006

Platinum nanoparticles protect HeLa cells from ROS damage.

The 19th International Meeting of Japanese Association for Animal Cell Technology (Kyoto)
Takeki Hamasaki et al.,

2006

Nordenau phenomenon-application of natural reduced water to therapy.
Follow up study upon 411 diabetes patients.

The 19th International Meeting of Japanese
Association for Animal Cell Technology (Kyoto)
Z. Gadek et al.,

2006

Action mechanisms of reduced water for prevention of oxidative stress-related diseases.

The satellite symposium of JAACT2006 Kyoto
(Fukuoka city)
Shirahata, S

2006

Application of water to medical care, fact and clinical application of study ofreduced water.

The Animal Cell Technology symposium of JAACT
2006 (Tokyo)
Shirahata Sanetaka

2006

Antioxidant activities and effects to Parkinson's disease model animals of platinum
nanoparticles and electrolyzed-reduced water supplemented with platinum nanoparticles.

Annual meeting of Japan Society for Bioscience,
Biotechnology, and Agrochemistry 2006
Eliko Takahashi et al.,

2006

Redox regulation by model water for electrolyzed-reduced water

The 8th Annual Meeting of the Japanese society for functional water
Ayumi Hosokawa et.al.

2009

Analysis of nano particle on biological availability and toxicity

The 8th Annual Meeting of the Japanese society for functional water
Keishi Kadooka et.al.

2009

Survival benefit of electrolyzed-reduced water on nematode

The 8th Annual Meeting of the Japanese society for functional water
H. Yan et.al.

2009

Analysis of inducible mechanism of cancer cell death by atomic hydrogen

The 8th Annual Meeting of the Japanese society for functional water
Katsuhiko Shigiyama et.al.

2009

Examination of anti-diabetic effect of model water for electrolyzed-reduced water

The 8th Annual Meeting of the Japanese society for functional water
Takuro Nakamura et.al.

2009

Suppressive effect of Electrolyzed Water with high dissolved Hydrogen (H2) on Cardiorenal syndrome of Dahl Salt sensitive rat

Renal Week 2009, American Society of Nephrology?
Wan-Jun Zhu et.al.

2009

Induction of apoptosis of cancer cells by atomic hydrogen produced by catalyst activity of platinum nanoparticles

The 68th Annual Meeting of the Japanese Cancer Association
Sanetaka Shirahata et.al.

2009

Use experience of electrolysis hemodialysis using electrolyzed-reduced water

The joint meeting of the 76th Annual Meeting of theHokkaido Dialysis Therapy and the 36th Scientific Meeting of the Tohoku renal failure
Susumu Uemura et.al.

2009

Clinical evaluation of hemodialysis using electrolyzed-reduced water

The 54th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2009)
Koichi Ishibashiri et.al.

2009

Case report of a patient accepted reduction of itch by dialysate using electrolyzed-reduced water

The 54th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2009)
Ryoichi Nakazawa et.al.

2009

Examination of hemodialysis system using electrolyzed water

The 54th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2009)
Hiroshi Nemoto et.al.

2009

Use experience of electrolysis hemodialysis using water at cathod side ofelectrolysis

The 54th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2009)
Susumu Uemura et.al.

2009

Effect of Electrolyzed-reduced water on skin temperature of lower limb on hemodialysis patients

The 54th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2009)
Akiko Kobayashi et.al.

2009

Evaluation of water from hemodialysis system by using electrolyzed-reduced water

The 54th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2009)
Minako Yataka et.al.

2009

Exploratory investigation about long-term clinical effect of electrolysishemodialysis

The 54th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2009)
Masaaki Nakayama et.al.

2009

Examination of intake of electrolyzed water on supressive effect of kidney injury in cronic kidney disease (CKD) model rat

The 52nd Annual Meeting of Japanese Society of Nephrology
Wan-Jun Zhu et.al.

2009

Fundamental investigation for supressive effect of intake of electrolyzed water on cardiorenal organ dysfunction

The 8th Scientific Meeting of the Japanese Society of Anti-Aging Medicine
Wan-Jun Zhu et.al.

2009

Biological effect of Electrolyzed Water in Hemodialysis

Nephron Clinical Practice 2009;112:c9–c15
Masaaki Nakayama et.al.

2009

Induction mechanism of cell death of cancer cells by atomic hydrogen

Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009
Katsuhiko Shigiyama et.al.

2009

Anti-diabetes effects of electrolyzed reduced water and its model water

Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009
Takuro Nakamura et.al.

2009

Redox regulation by electrolyzed reduced water and its model water

Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009
Ayumi Hosokawa et.al.

2009

Next-generation hemodialysis system for palliating lassitude after hemodaialysis

The 6th Japan Congress Support of Home Dialysis
Masaaki Nakayama et.al.

2009

Electrolyzed water at cathode side (EW) suppressed renal injury in chronic kidney disease rats

The 9th Scientific Meeting of renal failure pathosis therapy
Wan-Jun Zhu et.al.

2008

Collaboration for clinical effect of electrolysis hemodialysis

The 9th Scientific Meeting of renal failure pathosis therapy
Masaaki Nakayama et.al.

2008

Electrolyzed water at cathode side (EW) suppressed renal injury in chronic kidney disease rats

Renal Week 2008, American Society of Nephrology?
Wan-Jun Zhu et.al.

2008

Growth suppression of HL60 and L6 cells by atomic hydrogen

The 21st Annual and International Meeting of Japanese Association for Animal Cell Technology
K. Nakanishi et.al.

2008

Suppresive effects of hydrogen and atomic hydrogen on type 2 diabetes

The 21st Annual and International Meeting of Japanese Association for Animal Cell Technology
K. Haramaki et.al.

2008

Supressive effect of electrolyzed reduced water on lipid peroxidation and lipid absorption

The 21st Annual and International Meeting of Japanese Association for Animal Cell Technology
M. Abe et.al.

2008

Elongation of lifespan of nematode by electrolyzed reduced water

The 21st Annual and International Meeting of Japanese Association for Animal Cell Technology
H. Yan et.al.

2008

Inducible effect of cancer cell death by electrolyzed -reduced water and it's modelwater

The annual meeting of West Japan of Japan Society for Bioscience, Biotechnology, and Agrochemistry 2008
K. Kadekaru et.al.

2008

Antidiabetic effect of electrolyzed -reduced water and it's model water

The annual meeting of West Japan of Japan Society for Bioscience, Biotechnology, and Agrochemistry 2008
K. Haramaki et.al.

2008

Examination of hemodialysis system using electrolyzed water

The 36th Scientific Meeting of the Tohoku renal failure
Hiroshi Nemoto et.al.

2008

Investigation of biocompatibility about Hemodialysis solution (HD) by Electrolyzedwater (EW)

The 53th Annual Meeting of the Japanese Society for Dialysis Therapy
Masaaki Nakayama et.al.

2008

Protective effect of electrolyzed cathodic water on diabetic nephropathy

The 8th Scientific Meeting of the Japanese Society of Anti-Aging Medicine
Takefumi Mori et.al.

2008

Potential of intake of electrolyzed water (Alkaline ion water) in countermeasure for chronic kidney disease

The 8th Scientific Meeting of the Japanese Society of Anti-Aging Medicine
Masaaki Nakayama et.al.

2008

Intake of electrolyzed cathodic water reduce excretion of urine protein of streptozotocin-induced diabetes Dahl S rat.

The 51st Annual Meeting of Japanese Society of Nephrology
Yusuke Osaki et.al.

2008

Investigation of participation of methylglyoxal on Cardiorenal syndrome

Vascular Medicine 2008 vol.4 no.4 345-351
Masaaki Nakayama et.al.

2008

Kinetic analysis of superoxide anion radical-scavenging and hydroxyl radical-scavenging activities of platinum nanoparticles.

Langmuir, June, Web on line
Takeki Hamasaki et.al.

2008

Examination of electrolyzed-reduced water on survival benefit of nematode

Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry 2008
H. Yan et.al.

2008

Suppressive effect of Electrolyzed-reduced Water on Lipid peroxidation and absorption

Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry 2008
Masumi Abe et.al.

2008

Inhibitory Effect of Electrolyzed Reduced Water on Tumor Angiogenesis

Biological & Pharmaceutical Bulletin 31(1),19-26, January
Jun YE et.al.

2008

Intake of water with high levels of dissolved hydrogen produced by electrolysissuppresses ischemia-induced cardio-renal injury in Dahl salt sensitive rats

Nephrology Dialysis Transplantation Dec 30th,2010 Web online
Wan-Jun Zhu et.al.

2010

Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes Mellitus

Cytotechnology Nov 10th, 2010 Web online
Yupin Li et.al.

2010

Extension of the Lifespan of Caenorhabditis Elegans by Electrolyzed ReducedWater

Bioscience, Biotechnology, and Biochemistry 74(10),2011-2017,2010
Hankxu Yan et.al.

2010

Antioxidative effect of platinum nano particle on cultured animal cells

The 8th Annual Meeting of the Japanese society for functional water
Hidekazu Nakanishi et.al.

2010

Examination of mechanism of sugar transport signal activation by model water forelectrolyzed-reduced water

The 8th Annual Meeting of the Japanese society for functional water
Takeki Hamasaki et.al.

2010

Extension mechanism of the Lifespan of Caenorhabditis Elegans by ElectrolyzedReduced Water

The 8th Annual Meeting of the Japanese society for functional water
Tomoya Kinjou et.al.

2010

Hydrogen concentration and electrolyzed water-hemodialysis

The 55th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2010)
Ryoichi Nakazawa et.al

2010

Evaluation of blood pressure on electrolyzed water-hemodialysis

The 55th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2010)
Susumu Uemura et.al

2010

Development of new hemodialysis system by adding hydrogen(H2) to dialysate

The 53rd Annual Meeting of Japanese Society of Nephrology
Masaaki Nakayama et.al.

2010

Suppressive effect of drinking hydrogen dissolved water by water electrolysis on cardio-renal injury in CKD

The 10th Scientific Meeting of the Japanese Society of Anti-Aging Medicine
Wan-Jun Zhu et.al.

2010

A novel therapy suppressing oxidative stress and inflammation by hydrogen dissolved dialysate - hemodialysis system applied water electrolysis

The 10th Scientific Meeting of the Japanese Society of Anti-Aging Medicine
Masaaki Nakayama et.al.

2010

A novel bioactive hemodialysis system using dissolved dihydrogen(H2) produced by water electrolysis: A clinical trial

Nephrology Dialysis Transplantation Apr 12th,2010 Web online
Masaaki Nakayama et.al.

2010

Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis

Nephrology Dialysis Transplantation Feb 26th,2010 Web online
Kuo-Chin Huang et.al.

2010

Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells.

Free Radical Research 2010 Jan;44(1):101-7.
Noriko Miyazawa et.al.

2010

Advanced research on the health benefit of reduced water

Trends in Food Science and Technology Web online
Sanetaka Shirahata et.al.

2011

The neuroprotective effects of electrolyzed reduced water and its model watercontaining molecular hydrogen and Pt nanoparticles

BMC Proceedings 2011, 5(Suppl 8):P69
Hanxu Yan et.al.

2011

Anti-diabetes effect of water containing hydrogen molecule and Pt nanoparticles

BMC Proceedings 2011, 5(Suppl 8):P18
Sanetaka Shirahata et.al.

2011

Protective Effect of Electrolyzed Water with High Dissolved Hydrogen (H2) on the Development of Cardiorenal Syndrome by Aging in Dahl Salt Sensitive Rat

Kidney Week 2011, American Society of Nephrology
Wan-Jun Zhu et.al.

2011

Protective Effect of Electrolyzed Water with High Dissolved Hydrogen (H2) on the Development of Cardiorenal Syndrome by Aging in Dahl Salt Sensitive Rat

Kidney Week 2011, American Society of Nephrology
Wan-Jun Zhu et.al.

2011

Protective Effect of Electrolyzed Water with High Dissolved Hydrogen (H2) on the Development of Cardiorenal Syndrome by Aging in Dahl Salt Sensitive Rat

The 13th China-Japan Joint Hypertension Symposium
Wan-Jun Zhu et.al.

2011

The mechanism analysis of the antioxidative effect by water containing hydrogen molecule or Pt nanoparticles

The 10th Annual Meeting of the Japanese society for functional water
Tomoya Kinjou et.al.

2011

Analysis of the cell death induction effect of model water for electrolyzed reduced water on human promyelocytic leukemia HL-60 cells

The 10th Annual Meeting of the Japanese society for functional water
T. Hamasaki et.al.

2011

Protective effect of electrolyzed water with high dissolved hydrogen (H2) on the development of cardiorenal syndrome by aging in dahl salt sensitive rat

The 10th Annual Meeting of the Japanese society for functional water
Wan-Jun Zhu et.al.

2011

Electrolyzed Water-Hemodialysis - Development of built-in production system for reversed osmosis purified electrolyzed water-

The 20th Annual Meeting of the Japanese Society for Next Generation of Artificial Kidney
Shigeru Kabayama et.al.

2011

Mechanism of the Lifespan Extension of Caenorhabditis elegans by Electrolyzed Reduced Water—Participation of Pt Nanoparticles

Bioscience, Biotechnology, and Biochemistry 75 (7), 110072-1–5, 2011
Hankxu Yan et.al.

2011

Examination of Blood Pressure on Electrolyzed Water Hemodialysis

The 56th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2011)
Yoshie Mori et.al.

2011

Evaluation of nutrition on Electrolyzed Water-Hemodialysis

The 56th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2011)
Susumu Uemura et.al.

2011

Hydrogen containing peritoneal dialysis solution

The 56th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2011)
Shigeru Kabayama et.al.

2011

Examination of concentration of hydrogen on Electrolyzed Water-Hemodialysis

The 56th Annual Meeting of the Japanese Society for Dialysis Therapy (Japan Kidney Week 2011)
Yoichi Shimizu et.al.

2011

Intake of water with high levels of dissolved hydrogen produced by electrolysis suppresses ischemia-induced cardio-renal injury in CKD rats

The 54th Annual Meeting of Japanese Society of Nephrology
Wan-Jun Zhu et.al.

2011

Intake of electrolyzed water containg hydrogen suppresses age-related cardio-renal injury in CKD

The 11th Scientific Meeting of the Japanese Society of Anti-Aging Medicine
Wan-Jun Zhu et.al.

2011

Water That Works

Thursday, June 11, 2015

Medical Studies on Electrolyzed Reduced Water

Abstract

Highlights

Keywords

PubMed Results

Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein.

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PubMed Results

Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein.

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